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tumor-mutational-burden

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Calculates tumor mutational burden from WES/WGS/panel data with Friends of Cancer Research harmonization equations, per-assay calibration (FDA 10/Mb = 7.8 TSO500 = 8.4 OncomineTML), synonymous/indel/germline filtering, hypermutator tiering, blood TMB, and integration with HLA-LOH and neoantigen quality (Luksza 2017 fitness). Use when assessing ICI eligibility under tumor-specific cutoffs (McGrail 2021), comparing tissue vs bTMB, or auditing TMB-H reporting against ESMO 2024 and FDA pembrolizumab pan-tumor 2020.

When to use

• Calculate TMB from somatic VCF
• Apply ICI eligibility cutoffs
• Per-assay TMB calibration
• Synonymous/indel/germline filtering
• Hypermutator tiering
• Blood TMB calculation
• Integration with HLA-LOH
• Neoantigen quality integration

When not to use

• Comparing TMB across panels without per-assay calibration

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