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bio-splicing-quantification

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Quantifies alternative splicing events (PSI/percent spliced in) from RNA-seq using SUPPA2 from transcript TPM or rMATS-turbo from BAM files. Calculates inclusion levels for skipped exons, alternative splice sites, mutually exclusive exons, and retained introns. Use when measuring splice site usage or isoform ratios from RNA-seq data.

When to use

• measuring splice site usage
• measuring isoform ratios
• calculating inclusion levels for skipped exons
• calculating inclusion levels for alternative splice sites
• calculating inclusion levels for mutually exclusive exons
• calculating inclusion levels for retained introns
• quantifying splicing from RNA-seq

When not to use

• differential splicing comparison between conditions

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