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bio-splicing-pipeline

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End-to-end alternative splicing analysis from FASTQ to differential splicing results. Aligns with STAR 2-pass mode, performs junction QC, runs rMATS-turbo for differential analysis, and generates sashimi visualizations.

When to use

• comprehensive splicing analysis from raw RNA-seq data
• alternative splicing analysis
• differential splicing results
• STAR 2-pass alignment
• junction QC
• rMATS-turbo differential analysis
• sashimi visualizations
• IsoformSwitchAnalyzeR

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