domains / families / science / mirdeep2-analysis

mirdeep2-analysis

separate

Discovers novel miRNAs and quantifies known miRNAs with miRDeep2 by scoring genome-mapped read stacks against the Dicer/Drosha biogenesis signature. Use when deciding whether a study needs de novo discovery at all versus known-miRNA quantification; choosing the species and related-species miRBase references; reading the miRDeep2 score as a signal-to-noise hypothesis rather than a fixed cutoff; or filtering novel candidates against tRNA/rRNA loci to reject the classic false positives.

When to use

• Discover novel miRNAs in an animal genome
• Quantify known miRNAs
• Choosing species and related-species miRBase references
• Reading miRDeep2 score as a signal-to-noise hypothesis
• Filtering novel candidates against tRNA/rRNA loci

When not to use

• Quantify known miRNAs + isomiRs + tRFs on a supported species
• Profile tRFs / piRNAs
• Plant small RNAs
• Animal with NO genome assembly

Skill page

Source