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differential-mirna

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Tests miRNAs for differential expression with DESeq2 or edgeR using small-RNA-aware normalization and filtering. Use when deciding which normalization survives a library dominated by a few hyper-abundant miRNAs (compositional fragility); choosing DESeq2 vs edgeR vs a compositional method; setting a lower prefilter than mRNA; handling biofluid data with no endogenous normalizer; or remembering that RPM is for display and TDMD can make a miRNA drop without transcriptional repression.

When to use

• differential miRNA expression testing
• choosing normalization for hyper-abundant miRNAs
• selecting DESeq2 vs edgeR vs compositional methods
• setting lower prefilter thresholds than mRNA
• handling biofluid data without endogenous normalizers
• interpreting miRNA drops with TDMD
• isomiR-level differential expression

When not to use

• mRNA differential expression
• using RPM for statistical testing
• silently summing 5' isomiRs into mature counts

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