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reverse-complement

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Generate reverse complements and complements of DNA/RNA sequences using Biopython, including IUPAC ambiguity codes, gapped alignments, and minus-strand features. Use when working with the opposite strand, building reverse primers, normalizing strand orientation before alignment, or extracting a coding sequence from a minus-strand feature.

When to use

• working with the opposite strand
• building reverse primers
• normalizing strand orientation before alignment
• extracting a coding sequence from a minus-strand feature
• generating reverse complements of DNA sequences
• generating complements of DNA sequences
• generating reverse complements of RNA sequences
• generating complements of RNA sequences

When not to use

• hand-rolling the complement table
• reimplementing Biopython's reverse_complement
• transcribing the coding strand without complementing

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