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riboseq-pipeline

separate

End-to-end Ribo-seq analysis from FASTQ through periodicity QC, P-site calibration, ORF detection, translation efficiency, and stalling. Use when orchestrating a full ribosome profiling pipeline and deciding harvest/dedup/alignment options and which downstream analyses the library can support.

When to use

• orchestrating a full ribosome profiling pipeline
• deciding harvest/dedup/alignment options
• determining which downstream analyses the library can support
• UMI handling, trimming, rRNA depletion
• footprint-aware alignment
• periodicity QC
• P-site calibration
• ORF detection and differential translation

When not to use

• internals of any one step

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