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ribosome-periodicity

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Validate Ribo-seq library quality by measuring 3-nucleotide periodicity and calibrating read-length-specific P-site offsets. Use when checking whether footprints capture genuine translation, determining P-site offsets for downstream ORF/TE/stalling analysis, or deciding which read lengths to keep.

When to use

• measuring 3-nucleotide periodicity
• calibrating read-length-specific P-site offsets
• checking whether footprints capture genuine translation
• determining P-site offsets for downstream ORF/TE/stalling analysis
• deciding which read lengths to keep
• computing frame-0 fraction
• generating metaheatmap and metaprofile visualizations

When not to use

• gene-level counting for RNA-seq-like libraries
• codon-resolution analysis for libraries without periodicity

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