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initiation-site-mapping

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Map translation initiation sites, including non-AUG and alternative starts, from initiation-drug ribosome profiling (TI-seq). Use when locating start codons, detecting near-cognate or upstream initiation, or analyzing harringtonine, lactimidomycin (GTI-seq/QTI-seq), or retapamulin (Ribo-RET) data.

When to use

• locating start codons
• detecting near-cognate or upstream initiation
• analyzing harringtonine data
• analyzing lactimidomycin (GTI-seq/QTI-seq) data
• analyzing retapamulin (Ribo-RET) data
• TIS detection from harringtonine/LTM data
• cryptic/near-cognate starts detection
• bacterial initiation analysis

When not to use

• elongation ORF detection
• inferring start codons from elongation periodicity without initiation-drug library

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