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enzyme-selection

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Select restriction enzymes for cloning or diagnostics using Biopython Bio.Restriction. Finds enzymes by cut frequency, overhang type, recognition-site length, commercial availability, compatible ends, and methylation sensitivity, and identifies isoschizomers and compatible pairs. Use when choosing which enzymes to use to linearize a vector, drop in an insert, set up a diagnostic digest, or pick a methylation-insensitive enzyme.

When to use

• linearize a vector
• drop in an insert
• set up a diagnostic digest
• pick a methylation-insensitive enzyme
• find isoschizomers
• identify compatible pairs
• directional cloning
• find compatible ends

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