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bio-read-qc-umi-processing

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Extract, process, and deduplicate reads using Unique Molecular Identifiers (UMIs) with umi_tools. Use when library prep includes UMIs and accurate molecule counting is needed, such as in single-cell RNA-seq, low-input RNA-seq, or targeted sequencing to distinguish PCR from biological duplicates.

When to use

• single-cell RNA-seq
• low-input RNA-seq
• targeted sequencing
• accurate molecule counting
• PCR duplicate removal

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