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bio-primer-design-primer-validation

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Validate PCR primers for specificity, dimers, hairpins, and secondary structures using primer3-py thermodynamic calculations. Check self-complementarity, heterodimer formation, and 3' stability.

When to use

• Validate PCR primers
• Check hairpin formation
• Check self-dimer (homodimer)
• Check primer-primer dimer (heterodimer)
• Check 3' end stability
• Validate primer pair
• Calculate end stability

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