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off-target-prediction

separate

Nominates and assesses CRISPR off-target sites genome-wide. Enumerates candidate sites by mismatch and bulge tolerance with Cas-OFFinder/CRISPRitz, ranks them with the published CFD score (SpCas9-only, relative ranker) or MIT/CRISTA/energy models, runs variant-aware screening against gnomAD/individual genomes (CRISPRme), and frames the empirical genome-wide discovery assays (GUIDE-seq, CIRCLE-seq, CHANGE-seq, DISCOVER-seq, Digenome-seq) and high-fidelity nuclease choice (HiFi Cas9, Sniper-Cas9, eSpCas9, SpCas9-HF1). Use when assessing guide RNA specificity, choosing among candidate guides, screening a therapeutic guide against population variation, or planning empirical off-target validation. Distinguishes predicted vs detected vs validated. On-target activity scoring and deaminase (Cas-independent) base/prime-editor off-targets are separate skills.

When to use

• assessing guide RNA specificity
• choosing among candidate guides
• screening a therapeutic guide against population variation
• planning empirical off-target validation
• enumerating candidate sites by mismatch and bulge tolerance
• ranking sites with CFD or MIT/CRISTA/energy models
• variant-aware screening against gnomAD or individual genomes

When not to use

• on-target activity scoring
• deaminase (Cas-independent) base/prime-editor off-targets

Skill page

Source