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bio-genome-engineering-off-target-prediction

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Predict CRISPR off-target sites using Cas-OFFinder and CFD scoring algorithms. Identify potential unintended cleavage sites genome-wide and assess guide specificity. Use when evaluating guide RNA specificity or selecting guides with minimal off-target risk.

When to use

• evaluating guide RNA specificity
• selecting guides with minimal off-target risk
• searching genome for potential unintended cleavage sites
• scoring off-targets by cutting frequency determination (CFD)

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