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bio-long-read-sequencing-nanopore-methylation

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Calls DNA methylation from Oxford Nanopore sequencing data using signal-level analysis. Use when detecting 5mC or 6mA modifications directly from nanopore reads without bisulfite conversion.

When to use

• Extract 5mC/6mA modification probabilities
• Summarize per-site methylation frequencies
• Basecalling with methylation
• Region-specific analysis
• Sample summary
• Differential methylation

When not to use

• Bisulfite conversion

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