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13c-metabolic-flux

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Estimates intracellular metabolic fluxes from steady-state carbon-13 isotope-tracing measurements using validated atom maps, mfapy isotope simulation, constrained multistart fitting, and flux-profile diagnostics. Use for 13C-MFA, carbon tracing, mass isotopomer distributions (MDVs/MIDs), positional isotopomers, parallel tracer experiments, and determining whether labeling data constrain a pathway flux. Distinguishes measured-label inference from COBRA flux balance analysis and flags experiments requiring nonstationary MFA.

When to use

• 13C-MFA
• carbon tracing
• mass isotopomer distributions (MDVs/MIDs)
• positional isotopomers
• parallel tracer experiments
• determining whether labeling data constrain a pathway flux

When not to use

• COBRA flux balance analysis
• nonstationary MFA
• time-course labeling
• reconstructing isotope balances
• fitting each reaction independently

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