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bio-imaging-mass-cytometry-data-preprocessing

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Load and preprocess imaging mass cytometry (IMC) and MIBI data. Covers MCD/TIFF handling, hot pixel removal, and image normalization. Use when starting IMC analysis from raw MCD files or preparing images for segmentation.

When to use

• Load MCD files
• Apply hot pixel removal
• Channel cropping
• Signal normalization
• Prepare multiplexed images for segmentation
• Spillover correction
• Estimate spillover from single-stain controls
• Convert MCD to TIFF

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