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bio-genome-engineering-grna-design

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Design guide RNAs for CRISPR-Cas9/Cas12a experiments using CRISPRscan and local scoring algorithms. Score guides for on-target activity using Rule Set 2 and Azimuth models. Use when designing sgRNAs for gene knockout, activation, or repression experiments.

When to use

• Designing sgRNAs for gene knockout
• Designing sgRNAs for gene activation
• Designing sgRNAs for gene repression
• Scanning target gene sequences for PAM sites
• Extracting candidate spacer sequences
• Scoring guides for on-target activity

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