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doublet-detection

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Detects and removes doublets/aggregates from flow, spectral, and mass cytometry before clustering or quantification. Covers FSC-A vs FSC-H singlet discrimination (the Area-Height non-proportionality, not a 1D area gate), FSC-W/SSC width gating, CyTOF Gaussian discrimination parameters (Center/Offset/Width/Residual/Event_length) and DNA intercalator gating, and the residual heterotypic conjugates that survive scatter gating and masquerade as double-positive populations. Use when filtering aggregates before phenotyping, choosing a doublet method for flow vs CyTOF, or diagnosing a suspicious double-positive cluster.

When to use

• filtering aggregates before phenotyping
• choosing a doublet method for flow vs CyTOF
• diagnosing a suspicious double-positive cluster
• FSC-A vs FSC-H singlet discrimination
• FSC-W/SSC width gating
• CyTOF Gaussian discrimination parameters
• DNA intercalator gating
• residual heterotypic conjugates

When not to use

• scRNA-seq droplet methods
• DoubleFinder
• Scrublet
• scDblFinder

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