domains / families / science / bio-rna-quantification-featurecounts-counting

bio-rna-quantification-featurecounts-counting

separate

Count reads per gene from aligned BAM files using Subread featureCounts. Use when processing BAM files from STAR/HISAT2 to generate gene-level counts for DESeq2/edgeR.

When to use

• Count reads per gene from aligned BAM files
• Generate gene-level counts for DESeq2/edgeR
• Process BAM files from STAR/HISAT2
• Count reads mapping to genomic features
• Handle paired-end data counting
• Handle strand-specific libraries
• Count at transcript level
• Handle multi-mapping reads

Skill page

Source