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relion

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Validates and executes RELION single-particle cryo-EM refinement and half-map postprocessing. Supports STAR optics/acquisition checks, particle-stack consistency, gold-standard half sets, soft-mask validation, diagnostic Fourier shell correlation, and restart guidance.

When to use

• RELION single-particle refinement
• STAR optics and acquisition checks
• Particle-stack consistency validation
• Gold-standard half-set refinement
• Soft-mask validation
• Diagnostic Fourier shell correlation
• Restart guidance
• Half-map postprocessing

When not to use

• Motion correction
• Particle picking
• 2D/3D selection
• Biological interpretation of map quality
• Tomography
• Helical reconstruction
• Blush
• Heterogeneous-state modeling

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