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drugz-chemogenomic

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Analyzes CRISPR drug-modifier (chemogenomic) screens with drugZ (Colic et al. 2019 Genome Med), a bidirectional Z-score method that identifies synthetic-lethal sensitizing genes and resistance-conferring suppressor genes from vehicle vs drug comparisons. Covers vehicle-anchored design (not Day-0), the bidirectional Z math giving greater sensitivity to small-effect hits than MAGeCK / STARS / edgeR / RIGER on drug screens, per-gene sumZ and normZ, synth (sensitizer) vs supp (suppressor) FDR, multi-dose handling, integration with control sgRNAs, and comparison with MAGeCK MLE with dose covariate. Use when running a drug-modifier CRISPR screen, identifying sensitizing or resistance genes for a drug candidate, choosing drugZ vs MAGeCK MLE for chemogenomic analysis, troubleshooting low-effect drug screens where MAGeCK lacks sensitivity, or designing a drug-screen layout (vehicle vs drug arms).

When to use

• running a drug-modifier CRISPR screen
• identifying sensitizing or resistance genes for a drug candidate
• choosing drugZ vs MAGeCK MLE for chemogenomic analysis
• troubleshooting low-effect drug screens where MAGeCK lacks sensitivity
• designing a drug-screen layout (vehicle vs drug arms)
• multi-dose handling
• integration with control sgRNAs

When not to use

• Day-0 baseline comparisons
• general essentiality screens
• standard 2-condition essentiality analysis

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