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bio-crispr-screens-hit-calling

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Statistical methods for calling hits in CRISPR screens. Covers MAGeCK, BAGEL2, drugZ, and custom approaches for identifying essential and resistance genes. Use when identifying significant genes from screen count data after QC passes.

When to use

• Identifying essential genes from CRISPR screens
• Identifying resistance genes from CRISPR screens
• BAGEL2 Bayesian gene essentiality analysis
• DrugZ drug screen analysis
• Custom z-score hit calling
• Robust Rank Aggregation (MAGeCK-style)
• Second-Best sgRNA method

When not to use

• QC of screen count data

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