domains / families / science / alignment-validation

alignment-validation

separate

Validate alignment quality with insert size distribution, proper pairing rates, GC bias, strand balance, and other post-alignment metrics. Use when verifying alignment data quality before variant calling or quantification.

When to use

• Check alignment quality
• Compute post-alignment QC metrics
• File integrity validation
• Sequence dictionary identity check
• Contamination and sample swap detection
• Insert size distribution analysis

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