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bead-normalization

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Bead-based signal normalization and cross-batch harmonization for CyTOF and high-parameter cytometry - EQ four-element bead normalization of instrument sensitivity drift (CATALYST normCytof, premessa), and reference-anchor cross-batch normalization (CytoNorm, per-cluster quantile splines). Covers the distinction between within-run drift correction and between-batch correction, the mandatory anchor/reference sample, why normalization is per-cluster with many quantiles, and the over-correction risk. Use when correcting CyTOF signal drift, harmonizing multi-batch or multi-site studies, or deciding whether to normalize data versus model batch in the design.

When to use

• correcting CyTOF signal drift
• harmonizing multi-batch or multi-site studies
• deciding whether to normalize data versus model batch in the design
• EQ four-element bead normalization
• reference-anchor cross-batch normalization
• within-run drift correction
• between-batch correction

When not to use

• fixing staining-batch or reagent-lot effects with beads
• fixing intra-run detector drift with CytoNorm

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