domains / families / science / bio-basecalling

bio-basecalling

separate

Convert raw Nanopore signal data (FAST5/POD5) to nucleotide sequences using Dorado basecaller. Covers model selection, GPU acceleration, modified base detection, and quality filtering. Use when processing raw Nanopore data before alignment. Guppy is deprecated; use Dorado for all new analyses.

When to use

• Convert raw Nanopore signal data to nucleotide sequences
• Model selection for basecalling
• GPU acceleration
• Modified base detection
• Quality filtering
• Processing raw Nanopore data before alignment
• POD5 file handling
• Basecalling QC

When not to use

• Guppy for new analyses

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