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atac-qc

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ATAC-seq library quality control -- TSS enrichment, FRiP, fragment-size periodicity, library complexity (NRF/PBC1/PBC2), mitochondrial fraction, and ENCODE 4 thresholds. Use when assessing whether an ATAC-seq library passes ENCODE acceptance criteria, diagnosing transposition artefacts, comparing Omni-ATAC vs standard prep quality, or selecting which replicates to drop before peak calling.

When to use

• assessing whether an ATAC-seq library passes ENCODE acceptance criteria
• diagnosing transposition artefacts
• comparing Omni-ATAC vs standard prep quality
• selecting which replicates to drop before peak calling
• computing TSS enrichment
• computing FRiP
• computing fragment-size periodicity
• computing library complexity (NRF/PBC1/PBC2)

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