domains / families / science / bio-genome-assembly-assembly-polishing

bio-genome-assembly-assembly-polishing

separate

Polish genome assemblies to reduce errors using short reads (Pilon), long reads (Racon), or ONT-specific tools (medaka). Essential for improving long-read assembly accuracy.

When to use

• Polishing genome assemblies
• Reducing assembly errors
• Improving long-read assembly accuracy
• Using Pilon for Illumina polishing
• Using medaka for ONT assemblies
• Using Racon for quick long-read polishing
• Executing multi-round polishing workflows

When not to use

• De novo genome assembly
• Read alignment without polishing
• Variant calling
• Sequence quality control

Skill page

Source