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wgcna-analysis

separate

Use when building a weighted gene co-expression network from a bulk expression matrix and a sample group file, filtering variable genes by MAD, identifying co-expression modules with WGCNA, correlating modules with traits, and exporting module-level plots and gene tables. NOT for single-cell RNA-seq, differential expression testing, methylation analysis, or datasets that are too small for WGCNA after quality control.

When not to use

• Do not use for single-cell RNA-seq matrices.
• Do not use for methylation data, DEG testing, or other non-WGCNA workflows.
• Do not use if the user only wants exploratory discussion and does not want the analysis executed.
• Do not proceed if the dataset is obviously too small for WGCNA or becomes too small after QC.

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